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All About Pregaplan Ovulation Strip

Pregaplan is the single-step ovulation test kit, which lets you check whether you are ovulating. This test is a rapid chromatographic immunoassay for the detection of the LH hormone in urine. LH is Luteinizing Hormone that aids in the early ovulation detection.


Ovulation is the process of egg’s release from the ovary. This egg then passes into the fallopian tube, where it is ready for fertilization. For a successful pregnancy, the egg must fertilize within 24 hours of its release. Just before ovulation, the body produces the LH surge (i.e. large amounts of Luteinizing Hormone). Usually the hormone surge happens in the middle of your menstrual cycle. This LH is responsible for triggering the process of ovulation.
Pregaplan Test is a complete system to assist you in predicting the time of your peak fertility levels (i.e. the time when you are most likely to ovulate). During this time of maximum fertility, chances of conception are highest for the woman. The Pregaplan test detects the LH surge in the urine sample, indicating the occurrence of ovulation in the next 24 to 36 hours.


Working Mechanism:

Pregaplan Ovulation Test Kit works on the basic principle of detection of Luteinizing Hormone, by visually interpreting the color development on the device. The test region of the membrane clogs the anti-LH antibodies, whereas the control region locks-up the anti-mouse antibodies.
While testing, the urine sample reacts with anti-LH antibody, conjugated to the colored particles and pre-coated on the sample pad. The reacted mixture migrates via the membrane, and interacts with reagents on membrane. If the sample has sufficient amount of LH, a colored band forms in the test region. This colored band in membrane signifies a positive result. The display of a colored band in control region indicates a procedural control, which is a sign of addition of proper volume of specimen.

Sample Collection:

One should not use the first morning urine sample as LH synthesis in the body takes place early morning. Therefore, it will not show up in urine until later in day. The most suitable time for collecting samples is anytime between ten in morning to eight in evening. Collect samples at the same time every day. Lessen your liquid intake almost two hours before sample collection. This is because diluted urine samples might prevent the test from detecting the LH surge.

Testing Procedure:

Keep the Pregaplan plan test device, the urine sample and the control solution, all at normal room temperature. Open the test kit to find the ovulation device and the dropper. Draw about 3 drops of urine sample into the dropper. Dispense it properly on the device cassette. Now wait for the colored line to appear. You may read the results in around 10 minutes. Do not decipher the results after 15 minutes.

Result Interpretation:

Positive: The result is positive if two color bands are visible. The test band color line (T) is equal to or darker than the control band color line (C). A positive result means you will ovulate in the next 24-48 hours, and that is the most probable time to conceive.
Negative: The result will be negative either if only one color band appears in the Control region, or if the test color band is lighter than that for the control band.
Invalid: The results are invalid if either there are no visible bands, or there is a test band without the control band. In any of these cases, you should repeat the test with a new kit.

Quality Control Check for Pregaplan:

The Pregaplan test contains a mechanism for procedural control. A colored line in the Control region (C) is an internally controlled procedure for confirming sufficient volume of specimen and adequate wicking of the membrane. This also proves using the correct procedural technique.
Positive results indicate the LH level in urine as sufficient to begin ovulating in the next 24-36 hours. Negative results imply no detection of LH surge, a case in which you should continue your daily testing.